Vertilcillium lecani commonly called as white halo fungus is found sprouting on the body of coffee green scale Coccus viridis.
It has been extensively studied against the pest in Lower Pulney Hills in Tamil Nadu.
The fungus is known to cause epizootic when the environmental conditions are favourable.
The fungus is multiplied on cheap media for large scale production.
Sorghum grains devoid of pesticide residues (40 g) is washed in potable water and transferred to conical flask (250 ml) and 15 ml of distilled water is added.
The conical flasks are plugged with cotton and autoclaved for 20 min at 15 psi.
The flasks are allowed to cool and taken to laminar flow chamber for inoculation.
From a clean uncontaminated mother culture in slant loopful quantities of V. lecani spores are transferred aseptically.
The flasks are incubated at room temperature. The spores are obtained in a fortnight.
